Catalog Number - 68104-10P-2

Cogent RP C4 Butyl is a reversed-phase HPLC and UHPLC stationary phase designed for the separation of hydrophobic to moderately hydrophobic compounds. Manufactured using a traditional butyl (C4) bonded phase on ultra-high purity silica and fully double end capped, it provides reproducible retention, excellent peak shape, and reduced secondary interactions. The shorter alkyl chain offers less retention than C8 or C18 phases, making it well suited for fast analyses, biomolecules, peptides, proteins, and method development where excessive retention is undesirable.

68104-10P-2 is a UHPLC column packed with 1.8 µm RP C4 Butyl particles featuring a 100 Å pore size in a 2.1 mm ID x 100 mm format. The 100 mm length provides increased resolution and peak capacity compared to shorter columns, while the 1.8 µm particle technology delivers exceptional efficiency and chromatographic performance. This column is specifically engineered for high-pressure UHPLC applications and is intended to take full advantage of UHPLC instrumentation capable of operating at the elevated pressures generated by sub-2 µm particles. Typical operating flow rates are approximately 0.2–0.4 mL/min. Because of the low flow rates required by 2.1 mm ID columns, any HPLC system used with this column must be capable of delivering accurate and reproducible low-flow performance.

Ideal for very hydrophobic and neutral compounds, this column offers high efficiency, excellent resolution, reduced solvent consumption, and increased laboratory productivity. The 2.1 mm ID format is particularly attractive for LC-MS applications due to reduced solvent usage and improved mass spectrometer compatibility. While compatible HPLC systems may be used in some applications, this column is designed primarily for UHPLC operation where the full benefits of sub-2 µm particle technology—including higher efficiency, greater peak capacity, faster separations, and superior chromatographic performance—can be realized.

Key Benefits

• RP C4 Butyl stationary phase

• Fully double end capped

• Traditional butyl bonded phase

• Ultra-high purity silica support

• Engineered for UHPLC applications

• 1.8 µm particle technology

• Exceptional column efficiency

• Increased peak capacity

• Higher resolution separations

• Reduced solvent consumption

Typical Applications

• UHPLC analysis

• High-pressure liquid chromatography

• LC-MS methods

• High-efficiency separations

• Method development

• Peptide analysis

• Protein analysis

• Hydrophobic compound analysis

• Neutral compound analysis

• Pharmaceutical analysis

Specifications

• Product Type: UHPLC Column

• Stationary Phase: RP C4 Butyl

• Chromatography Mode: Reversed Phase

• Bonded Phase: Butyl (C4)

• Endcapping: Fully Double End Capped

• Particle Size: 1.8 µm

• Pore Size: 100 Å

• Column Dimensions: 2.1 mm ID x 100 mm

• Length: 100 mm

• Typical Flow Rate: 0.2–0.4 mL/min

• Brand: Cogent

Important Notes

• Specifically designed for UHPLC operation and high-pressure reversed-phase separations

• Delivers maximum performance when used on a UHPLC system designed for sub-2 µm particle columns

• May be used on compatible HPLC systems that can reliably operate at the required pressures and low flow rates

• Sub-2 µm particles generate significantly higher backpressure than conventional HPLC columns

• The 2.1 mm ID format is ideal for LC-MS applications and reduced solvent consumption

• Standard HPLC systems must be capable of precise and reproducible operation at approximately 0.2–0.4 mL/min

• The 100 mm length provides increased resolution and peak capacity compared to shorter column formats

• Samples should be filtered through a 0.2 µm syringe filter prior to injection

• Lower retention than comparable C8 or C18 stationary phases

  ---